Figure 2. .
Effect of cPLA2α inhibitors (AACOCF3 and MAFP) on MIP-133–induced cPLA2α enzyme activity in HCE cells. HCE cells were preincubated for 1 hour with cPLA2α inhibitors (10 μM MAFP or 20 μM AACOCF3) or an inactive negative control (20 μM AACOCH3), and then incubated with or without 15 μg/mL MIP-133 for 24 hours. Induction of cPLA2α enzyme activity was examined using a cPLA2 assay kit. The data are mean ± SEM of three independent experiments. Asterisk indicates P value < 0.05 by unpaired Student's t-test.