Figure 3. .
Effect of anti–MIP-133 antiserum (ImS) on MIP-133–induced cPLA2α enzyme activity in HCE cells. HCE cells were preincubated for 1 hour with chicken anti–MIP-133 antiserum (ImS, 1:75 dilution) or chicken preimmune normal serum control (NS, 1:75 dilution), and then incubated with or without 15 μg/mL MIP-133 for 24 hours. Induction of cPLA2α enzyme activity was examined using a cPLA2 assay kit. The data are mean ± SEM of three independent experiments. Asterisk indicates P value < 0.05 by unpaired Student's t-test.