Figure 5. .
MIP-133–induced apoptosis is blocked by cPLA2α inhibitors in HCE cells. HCE cells were preincubated for 1 hour with cPLA2α inhibitors (10 μM MAFP or 20 μM AACOCF3) or an inactive negative control (20 μM AACOCH3), and then incubated with or without 15 μg/mL MIP-133 for 24 hours. Apoptosis was examined by Cell Death Detection ELISAPlus. MIP-133–induced apoptosis was confirmed by a positive control provided in the apoptosis kit. The data are mean ± SEM of three independent experiments. Asterisk indicates P value < 0.05 by unpaired Student's t-test.