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(A–D) MIP-133 upregulates IL-8 (A), IL-6 (B), IL-1β (C), and IFN-γ (D) mRNA in HCE cells. HCE cells were exposed to MIP-133 at doses of 7.5, 15, and 50 μg/mL for 6, 12, and 24 hours, and were then processed for total RNA isolation and RT-PCR analysis. The amount of mRNA expression was quantified by densitometry of bands in comparison to GAPDH. The experiment was repeated three times.
