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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Allergy Clin Immunol. 2014 Feb 13;133(5):1439–1447.e8. doi: 10.1016/j.jaci.2013.11.041

Figure 2. EMR1 is expressed late in eosinophil development.

Figure 2

(A,B) Surface expression of IL-5Rα, but not EMR1, is detected on CD34+ cells in bone marrow from a patient with HES A) and on purified CD34+ cells isolated from peripheral blood (B). (C-G) In vitro differentiation of CD34+ cells. Fast-green/neutral red staining of cultured CD34+ cells 2 days prior to (C) and 21 days after (D) the addition of IL-5. The arrow indicates a mature eosinophil with bilobed red nucleus and green secondary granules. Surface expression of EMR1 and CD34 as a function of time (E). mRNA expression as a function of time for EPX, MBP, and EDN (F; left axis), ECP (F; right axis), EMR1 and Siglec-8 (G; left axis) and IL-5Rα (G; right axis).