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. 2014 Jul 28;9(7):e103098. doi: 10.1371/journal.pone.0103098

Figure 5. Expression of key defence genes of B. napus at 7 or 14 dpi with either L. maculans ‘brassicae’ (Lmb) or L. biglobosa ‘canadensis’ (Lbc).

Figure 5

RNA-seq data for eight key Brassica defence genes were determined at 7 and 14 dpi. Average expression (FPKM) after infection by L. maculans (blue), L. biglobosa (red), and mock inoculum (green) is plotted. Error bars indicate the Cufflinks 95% confidence interval for each FPKM value. The genes assayed were 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), 1-amino-cyclopropane-1-carboxylate synthase 2 (ACS2), chitinase (CHI), hevein-like protein (HEL), isochorismate synthase 1 (ICS1), Pathogenesis related protein 1 (PR-1), WRKY transcription factor 70 (WRKY70), and plant defensin 1 (PDF1.2). Each gene was also classified according to hormone(s) abscisic acid (ABA), ethylene (ET), jasmonic acid (JA) and salicylic acid (SA) that induced higher expression.