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. 2014 Jul 28;9(7):e103118. doi: 10.1371/journal.pone.0103118

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© 2014 Torres-Fuentes et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Calcium influx in Hek293a (wild type) cells versus Hek-GHS-R1a-EGFP cells (A) and dose response curves of H. procumbens root extract (B) and ghrelin (C) are depicted. Exposure to ghrelin, the endogenous ghrelin receptor ligand, and H. procumbens root extract potently increase intracellular calcium through activation of the GHS-R1a receptor in a dose dependent manner. Calcium increase was depicted as a percentage of maximal calcium increase as elicited by control in each separate experiment (3.33% FBS). The data represents the mean ± SEM of a representative experiment out of three independent experiments with each concentration point performed in triplicate.