Figure 4. CPAF expression during acute and persistent infection.

HeLa, oviduct epithelial cells and MEFs were infected with C. trachomatis L2 at an MOI of 1 with or with addition of 100 U/ml PenG. Shown are representative Western Blots of uninfected (Control), acutely infected (Infection) or persistently (PenG) infected cells. Whole cell lysates were prepared with either RIPA (A) or UREA (B) extraction buffer (see methods) at 24, 40 and 48 h p.i. and 10 µg protein was loaded onto each lane. CPAFc: active CPAF; cHsp60: chlamydial Hsp60 protein; Actin: used as loading control.