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. 2014 Jul 28;9(7):e103061. doi: 10.1371/journal.pone.0103061

Figure 5. Real-time PCR of spatially upregulated genes that overlap between articular and growth plate cartilage zones.

Figure 5

Relative expression of selected genes that overlap between RZ and IDZ, including Bmp3, Grem1, and Sfrp5 (D–F), PZ and SZ, including Gdf10, Prelp, and Olfml3 (G–I), as well as HZ and SZ, including Adamts1, Mmp13, and Mmp9 (J–L), was determined in proximal tibial epiphyses of 10-day-old rats (n = 4) and values were normalized to 18S rRNA. Accuracy of the microdissection technique was validated by inspecting relative expression of Prg4, Col10a1, and Alpl (A–C). SZ, superficial zone; IDZ, intermediate/deep zone; RZ, resting zone; PZ, proliferative zone; HZ, hypertrophic zone; N.S., not significant; *P<0.05; **P<0.01; ***P<0.001.