Table 1.

ELISA anti-MUC1 and anti-T_helper antibody titers^[a] after three and four immunizations with various preparations.

Imm[b]	IgG total MUC1 Imm 3	IgG total MUC1 EP	IgG1 MUC1 EP	IgG2a MUC1 EP	IgG2b MUC1 EP	IgG3 MUC1 EP	IgM MUC1 EP	IgG total Thelper EP
1	28 500	59 100	17 800	7000	26 800	11 300	100	300
2	700	1000	400	0	600	200	50	1000
3	7800	10 600	9000	1000	6800	5300	50	50
4	0	0	0	0	0	0	50	50
IFA	0	0	0	0	0	0	100	50

^[a]Anti-MUC1 and anti-T_helper antibody titers are presented as median values for groups of mice. ELISA plates were coated with BSA-MI-CTSAPDT-(αGalNAc)RPAP conjugate for anti-MUC1 antibody titers or NeutrAvidin-biotin-T_helper for anti-T_helper antibody titers. Titers were determined by linear regression analysis, with plotting of dilution versus absorbance. Titers are defined as the highest dilutions yielding optical densities of 0.1 or greater relative to normal control mouse sera. [b] For immunizations with 1 and 2 liposomal preparations were employed, whereas 3 and 4 were given together with IFA. MT.MUC1 tumors were induced between the third and the fourth immunization. EP indicates endpoint serum samples.