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. 2014 Jul 21;9:3439–3452. doi: 10.2147/IJN.S65353

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© 2014 Song et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Detection of HIF-1α and induced gene expression.

Notes: Seven days after treatment, the gastrocnemius muscles in mice ischemic hindlimbs were harvested for Western blot and qPCR analysis. (A) Representative Western blot image for HIF-1α protein detection. (B) qPCR analysis of HIF-1α gene expression. *P<0.05 versus all other groups; ^#P<0.05 versus the saline group. (C) qPCR analysis of HIF-1α induced gene expression. *P<0.05 versus all other groups; ^#P<0.05 versus the saline group (n=7). Pluronic^® L64 (Sigma-Aldrich, St Louis, MO, USA).

Abbreviations: L64, Pluronic^® L64; qPCR, quantitative polymerase chain reaction; mRNA, messenger RNA; CMV, cytomegalovirus; SC, CMV promoter/SV40 enhancer; HIF-1α^tri, hypoxia-inducible factor 1-alpha triple mutant; VEGF, vascular endothelial growth factor; SDF-1, stromal cell-derived factor; PDGF-B, platelet derived growth factor; SV40E, SV40 enhancer.