Skip to main content
. Author manuscript; available in PMC: 2015 May 16.
Published in final edited form as: Nephron Exp Nephrol. 2014 May 16;126(3):141–147. doi: 10.1159/000362452

Figure 1. Id3-/- female mice develop kidney disease after 8 wks on a western diet.

Figure 1

WT or Id3-/- female mice were fed regular mouse chow (open symbols) or western diet (closed symbols). Number of mice in each group is indicated in parenthesis. Urine samples (24 hr) were analyzed for urinary albumin to creatinine ratios (ACR) (a). PAS stained kidney sections were studied for frequency of glomeruli affected (b) and severity of GN was scored (c). Kidney sections were stained by immunofluorescence for fibronectin deposition and results show percent glomerular area positive for fibronectin (d). Inflammatory cell infiltration in the glomeruli was quantified by measuring glomerular area staining positive for CD68+ macrophages (e). Each data point represents one mouse. Statistical analyses by ANOVA with Bonferroni post test for multiple comparisons. p values indicate statistical significance compared with wild type controls. Detailed methodology and results of stereologic analyses for fibronectin and CD68 positive staining are presented in Supplementary Table 2.