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. 2014 Jul 28;9(7):e103661. doi: 10.1371/journal.pone.0103661

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© 2014 Mich et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Design of the Gli-dependent nuclear mCherry reporter constructs. (B–C) Tg(8xGliBS:mCherry-NLS) and Tg(8xGliBS:mCherry-NLS-Odc1) larvae at 84 hpf. Fluorescence and brightfield images of representative zebrafish are shown, and the telencephalic nuclei (tn), optic tectum (ot), cerebellum (c), and hindbrain (hb) are labeled. Embryo orientations: lateral view and anterior left. Scale bars: 200 µm.