Table 3.

Mutation rates caused by pol30 mutations in the msh6Δ2-50 msh3Δ double mutant.

Genotype	hom3-10 Reversion Rate	lys2-10A Reversion Rate	CanR Mutation Rate
Wild-type
alone	2.26 [1.22–3.30] × 10−9 (1)	1.01 [0.67–1.86] × 10−8 (1)	5.65 [3.56–8.53] × 10−8 (1)
msh6Δ2-50 msh3Δ	3.47 [2.70–6.77] × 10−7 (154)	1.15 [0.73–2.99] × 10−6 (114)	4.57 [2.52–6.24] × 10−7 (8)
pol30-E143K
alone	5.38 [4.17–10.5] × 10−9 (2)	2.14 [1.33–2.86] × 10−7 (21)	9.94 [7.37–20.0] × 10−8 (1.8)
msh6Δ2-50 msh3Δ	9.60 [6.25–13.7] × 10−7 (425)	5.30 [3.47–5.59] × 10−6 (525)	5.99 [4.35–7.79] × 10−7 (11)
pol30-K217E
alone	9.27 [7.47–18.0] × 10−9 (4)	3.26 [2.39–5.33] × 10−7 (32)	3.99 [3.43–5.98] × 10−7 (7)
msh6Δ2-50 msh3Δ	3.20 [2.6–4.38] × 10−6 (1,416)	2.16 [1.74–3.50] × 10−5 (2,139)	1.93 [1.52–2.41] × 10−6 (34)
pol30-K13E
alone	5.69 [3.11–9.28] × 10−9 (2.5)	1.61 [1.21–2.33] × 10−7 (16)	3.10 [2.52–4.99] × 10−7 (5)
msh6Δ2-50 msh3Δ	1.95 [1.46–2.64] × 10−6 (863)	1.35 [0.71–1.74] × 10−5 (1,337)	1.27 [1.13–1.66] × 10−6 (22)
pol30-F254L
alone	2.83 [1.39–3.20] × 10−9 (1.3)	3.50 [2.26–4.85] × 10−8 (3.5)	9.76 [7.89–11.8] × 10−8 (1.7)
msh6Δ2-50 msh3Δ	4.59 [3.46–6.07] × 10−7 (203)	2.46 [1.67–3.6] × 10−6 (244)	4.57 [3.79–7.39] × 10−7 (8)
pol30-L68S
alone	4.31 [2.74–6.50] × 10−8 (19)	8.43 [7.14–10.8] × 10−7 (83)	5.00 [3.79–8.67] × 10−7 (9)
msh6Δ2-50 msh3Δ	1.51 [1.21–2.32] × 10−6 (668)	9.69 [7.42–14.4] × 10−6 (959)	1.78 [1.10–2.46] × 10−6 (32)

Reported rates are the median rates with 95% confidence interval in square brackets. Fold increase in mutation rate over the wild-type strain rate (RDKY5964) is listed in parenthesis.