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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Clin Cancer Res. 2013 Oct 4;20(1):131–139. doi: 10.1158/1078-0432.CCR-13-1016

Figure 4. IL-7, unlike IL-2, supports in vitro the proliferation and function of IL-7Rα.CAR-GD2+ EBV-CTLs in the presence of Tregs.

Figure 4

Panel A. IL-7Rα.CAR-GD2+ EBV-CTLs were co-cultured with CHLA-255 GFP-tagged cells (ratio 1:2) in the presence of IL-2 or IL-7, with or without Tregs. The percentage of residual tumor cells was measured by flow cytometry on day 7 of culture. The plots on the left show a representative experiment, while the graph on the right summarizes mean ± SD of 5 independent experiments. Panel B. IL-7Rα.CAR-GD2+ EBV-CTLs were labeled with CFSE and activated with autologous LCLs in the presence of IL-2 (upper plots) or IL-7 (lower plots) with or without Tregs. CFSE dilution was measured at day 7 of culture by flow cytometry. The plots on the left show a representative experiment, while the graph represents mean ± SD of 5 independent experiments. * p<0.01; ** p=0.005