Figure 5. FtsZ is required for EzrA localization to the midcell.

Strain PL851 encoding ezrA-gfp and an IPTG inducible ftsZ allele, ftsZ::P_spac-ftsZ, was grown in LB at 37°C for three generations in the presence (A) or absence of inducer (B). Depletion of FtsZ disrupts EzrA-GFP localization to midcell. Instead, EzrA-GFP appears diffusely distributed throughout the cell. Exposure times are the same in A and B, however brightness and contrast were increased in B to allow for better visualization of diffuse EzrA-GFP localization.