Table 1.
SNP | Primer 5′---3′ | Probe 5′---3′ | Annealing temperature |
---|---|---|---|
rs5443 | GNB3 Loaded-469F: | GNB3 probe 1 | 60°C |
5-TCCCACGAGAGCATCATCTG-3 | 5-Fam-CATCACGTCcGTGGCCTTCTC-TAMRA-3 | ||
GNB3 Loaded-548R: | GNB3 probe 2 | ||
5-TCGTCGTAGCCAGCGAATAGT-3 | 5-HEX-CATCACGTCtGTGGCCTTCTCC-TAMRA-3 | ||
| |||
rs4680 | COMT Loaded-471F: | COMT probe 1 | 60°C |
5- ATCACCCAGCGGATGGTGG-3 | 5-Fam-TTTCGCTGGCaTGAAGGACAA-TAMRA-3 | ||
COMT Loaded-531R: | COMT Probe2 | ||
5- ACGGGTCAGGCATGCACAC-3 | 5-HEX-TTTCGCTGGCgTGAAGGACA-TAMRA-3 |