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. 2014 Jul 19;20:1246–1254. doi: 10.12659/MSM.890315

Table 1.

GNβ3 and COMT polymorphism primers.

SNP Primer 5′---3′ Probe 5′---3′ Annealing temperature
rs5443 GNB3 Loaded-469F: GNB3 probe 1 60°C
5-TCCCACGAGAGCATCATCTG-3 5-Fam-CATCACGTCcGTGGCCTTCTC-TAMRA-3
GNB3 Loaded-548R: GNB3 probe 2
5-TCGTCGTAGCCAGCGAATAGT-3 5-HEX-CATCACGTCtGTGGCCTTCTCC-TAMRA-3

rs4680 COMT Loaded-471F: COMT probe 1 60°C
5- ATCACCCAGCGGATGGTGG-3 5-Fam-TTTCGCTGGCaTGAAGGACAA-TAMRA-3
COMT Loaded-531R: COMT Probe2
5- ACGGGTCAGGCATGCACAC-3 5-HEX-TTTCGCTGGCgTGAAGGACA-TAMRA-3