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. 2014 Jan 19;32(2):158–168. doi: 10.1038/nbt.2782

Figure 4. Deconvoluting the serum antibody repertoire.

Figure 4

B cells from peripheral blood or other tissues are sorted and subjected to high-throughput immunoglobulin V-gene sequencing, resulting in generation of a personal antibody sequence database. Antigen-specific antibodies from serum are then isolated by affinity chromatography, digested into peptides, and subjected to LC-MS/MS analysis. The MS/MS data are interpreted using the antibody sequence database, thereby allowing identification of CDRH3-derived peptides and the genes encoding the repertoire of antigen-specific antibodies in the serum. Ig, immunoglobulin.