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. 2014 Jul 14;8(3):1005–1009. doi: 10.3892/etm.2014.1844

Figure 1.

Figure 1

Effect of the recombinant plasmid targeting the Aurora-B gene on Aurora-B expression in HepG2 cells. (A) Analysis of the mRNA expression level of Aurora-B in HepG2 cells by quantitative polymerase chain reaction. The relative mRNA expression level of Aurora-B (Aurora-B/GAPDH) in the cells transfected with the recombinant plasmid was significantly lower compared with those transfected with the negative control plasmid. Data are presented as the mean ± standard deviation (n=6). *P<0.05, vs. negative control. (B) Analysis of Aurora-B protein expression levels in HepG2 cells by western blot analysis. Aurora-B protein expression in the cells transfected with the recombinant plasmid was significantly lower compared with those transfected with the negative control plasmid. GADPH, glyceraldehyde 3-phosphate dehydrogenase.