Table II.
Quantitative multiplexing protein analysis.
| No POPF, pg/mg total protein (n=16) | POPF, pg/mg total protein (n=15) | ||||||
|---|---|---|---|---|---|---|---|
|
|
|
||||||
| Analyte | Min | Median | Max | Min | Median | Max | P-valuea |
| IL-1β | 2.2 | 59.0 | 2427 | 13.2 | 71.3 | 1665 | 0.7529 |
| IL-2 | 2.44 | 2.4 | 318.7 | 2.4 | 2.4 | 1845 | 0.8615 |
| IL-4 | 4.7 | 9.6 | 16.7 | 4.6 | 9.2 | 16.7 | 0.8260 |
| IL-5 | 2.2 | 3.1 | 8.9 | 2.2 | 3.2 | 3.62 | 0.5990 |
| IL-6 | 1.7 | 301.3 | 11705 | 0.7 | 64.6 | 20730 | 0.0345 |
| IL-7 | 2.3 | 366 | 4579 | 36.9 | 1788 | 3116 | 0.3106b |
| IL-8 | 3.1 | 218 | 23614 | 2.7 | 46 | 1114 | 0.0029b |
| IL-10 | 2.3 | 8.2 | 26.9 | 69.5 | 881 | 1901 | <0.0001b |
| IL-12 | 3.1 | 331 | 1191 | 3.3 | 10.4 | 33,93 | 0.0045b |
| IL-13 | 50.0 | 90 | 4553 | 49.5 | 103.5 | 4283 | 0.9538 |
| IL-17 | 1.6 | 128.9 | 264.5 | 94.0 | 167.8 | 335 | 0.0399b |
| G-CSF | 1.3 | 301.3 | 2796 | 1.3 | 65 | 2414 | 0.6050 |
| IFN-γ | 0.0 | 370.9 | 12585 | 0.0 | 659.5 | 2714 | 0.3099 |
| GM-CSF | 560.4 | 898 | 2892 | 757.8 | 1057 | 1466 | 0.0570 |
| MCP-1 | 0.1 | 2882 | 34386 | 986.1 | 2901 | 23659 | 0.6334 |
| MIP-1β | 0.0 | 2033 | 8178 | 0.0 | 648.4 | 22307 | 0.1548 |
| TNF-α | 1.2 | 1.9 | 131.6 | 1.2 | 3.3 | 141 | 0.7136 |
| Angiopoietin-2 | 21.8 | 205.6 | 648.1 | 0 | 61.3 | 11684 | 0.1382 |
| Follistatin | 64.7 | 272.9 | 782.4 | 41.0 | 200.3 | 801.2 | 0.2949 |
| HGF | 128.6 | 3216 | 10962 | 132.4 | 3385 | 9905 | 0.8279 |
| PDGF-BB | 8.3 | 21.6 | 381.7 | 2.9 | 17.0 | 158.6 | 0.3365 |
| PECAM-1 | 10751 | 22656 | 22656 | 1528 | 18530 | 22656 | 0.2770 |
| VEGF | 3.6 | 38.5 | 226.4 | 1.0 | 49.2 | 176.8 | 0.3845 |
| C-peptide | 8.5 | 2691 | 2691 | 1.1 | 31.3 | 2691 | 0.0709 |
| Ghrelin | 2.0 | 11.6 | 485.9 | 2.0 | 71.2 | 287.8 | 0.1638 |
| GIP | 0.5 | 2.4 | 6.5 | 0.8 | 4.3 | 6.1 | 0.0225b |
| GLP-1 | 0.5 | 1053 | 1645 | 0.5 | 502.8 | 1409 | 0.0402b |
| Insulin | 0.3 | 2027 | 2027 | 2.6 | 2027 | 2027 | 0.4873 |
| Leptin | 7.9 | 92.1 | 406.6 | 5.4 | 75.9 | 248.5 | 0.1015 |
| PAI-1 | 130 | 1134 | 5712 | 37.2 | 421.8 | 2201 | 0.1149 |
| MMP-1 | 1.0 | 44.69 | 914.8 | 9.886 | 33.58 | 46.91 | 0.0452b |
| MMP-2 | 1473 | 15924 | 23015 | 47.07 | 3344 | 21497 | 0.0049b |
| MMP-3 | 20.24 | 997.5 | 5747 | 0 | 159.8 | 1144 | 0.0012b |
| MMP-7 | 92.55 | 124.6 | 1034 | 95.11 | 113.1 | 136.2 | 0.0880 |
| MMP-8 | 519.2 | 15319 | 88596 | 0 | 10490 | 169078 | 0.6494 |
| MMP-9 | 3904 | 41120 | 5.26×106 | 774 | 29880 | 5.45×106 | 0.9842 |
| MMP-12 | 189.0 | 197.8 | 238.7 | 3.249 | 191.9 | 218.2 | 0.0369b |
| MMP-13 | 82.84 | 105.9 | 240 | 73.6 | 103.6 | 333.5 | 0.9202 |
Concentrations of 38 wound healing analytes in the resection edge tissue following pancreatic surgery. The group without fistulas were compared with the fistula group using range, median and P-values.
Mann-Whitney U-test. The two groups included patients with pancreas head resections and distal pancreatectomy.
Statistically significant differences.
POPF, postoperative pancreatic fistula formation; IL, interleukin; G-CSF, granulocyte colony-stimulating factor; IFN, interferon; GM-CSF, granulocyte-macrophage colony-stimulating factor; MCP, monocyte chemotactic protein; MIP, macrophage inflammatory protein; TNF, tumor necrosis factor; HGF, hepatocyte growth factor; PDGF, platelet-derived growth factor; PECAM, platelet endothelial cell adhesion molecule; VEGF, vascular endothelial growth factor; GIP, gastric inhibitory polypeptide; GLP, glucagon-like peptide; PAI, plasminogen activator inhibitor; MMP, matrix metalloproteinase; Min, minimum; Max, maximum.