Table III.
IHC analysis of the frequency and validation of the biochemical parameters.
| IL-6 | IL-8 | VEGF | MMP-1 | MMP-2 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
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| Parameter | Inten | Distrib | Inten | Distrib | Inten | Distrib | Inten | Distrib | Inten | Distrib |
| POPF | ||||||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
| 1 | 3 | 3 | 0 | 1 | 3 | 3 | 4 | 4 | 5 | 1 |
| 2 | 5 | 7 | 6 | 9 | 5 | 7 | 5 | 5 | 4 | 9 |
| 3 | 2 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
| No POPF | ||||||||||
| 1 | 2 | 5 | 0 | 6 | 3 | 6 | 2 | 3 | 1 | 1 |
| 2 | 4 | 5 | 9 | 4 | 7 | 4 | 4 | 7 | 8 | 9 |
| 3 | 4 | 0 | 1 | 0 | 0 | 0 | 4 | 0 | 1 | 0 |
| P-valuea | 0.727 | 0.649 | 0.303 | 0.057 | 0.542 | 0.369 | 0.124 | 0.649 | 0.140 | 1.000 |
| P-valueb | 0.558 | 0.649 | 0.303 | 0.057 | 0.719 | 0.369 | 0.054 | 0.469 | 0.276 | 1.000 |
| Int. × distrba | 0.337 | 0.015 | 0.470 | 0.017 | 0.47 | |||||
Staining distributions and intensities were graded as described in the material and methods.
a
Fisher’s exact test;
b
exact Cochran-Armitage trend test in cases of three or four categories.
IL, interleukin; VEGF, vascular endothelial growth factor; MMP, matrix metalloproteinase; POPF, postoperative pancreatic fistula formation; Inten, intensity; Distrib, distribution; IHC, immunohistochemistry. The values in bold indicate statistically significant differences or close to being significant. Int × distrb is an additional scoring option in our analysis supporting the significance of the individual validations for intensity and for distribution.