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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: J Neuroimmunol. 2014 Jun 7;273(0):31–41. doi: 10.1016/j.jneuroim.2014.05.015

Figure 2. AS101 promotes IL-2 production by lymphocytes cultured under Th17 polarizing condition.

Figure 2

A. CD4+ T cells were cultured under Th17 (TGF-β, IL-6, and IL-23) polarizing conditions in the presence or absence of AS101 as described in Fig. 1A. Cell culture supernatants were harvested and analyzed by IL-2 ELISA (Biolegend). Compared to cells stimulated with Th17 polarizing cytokines, AS101 (0.5μg/ml) significantly increased production of IL-2 in Th17 cells (p<0.01). B. CD4+ T cells were cultured in the presence or absence of anti-IL-2 (20μg/ml) under Th17 polarization condition with AS101. Neutralization of IL-2 partially reduced the effects of AS101 on inhibition of IL-17 production. Summary of 3 individual experiments is shown (Mean ± SEM). P<0.05.