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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: J Neuroimmunol. 2014 Jun 7;273(0):31–41. doi: 10.1016/j.jneuroim.2014.05.015

Figure 5. AS101 inhibits activation of NFAT1, Akt, but increase activation of Erk1/2 in activated CD4+ T cells resulting in reduction of GM-CSF.

Figure 5

A. CD4+ T cells were cultured as described in Fig. 4. The cell lysates were used for Western blot to detect activation of ERK1/2, Akt, and NFAT1. One of 3 representative experiments is shown. B. CD4+ T cells were induced to produce GM-CSF as described in Fig. 4 with or without U0126 (20μM) and LY294002 (10μM). The GM-CSF producing cells were detected by flow cytometry. Results are summary of 3 representative experiments (Mean ± SEM). P< 0.05. C. CD4+ T cells were cultured as described in Fig. 5B. Western blots were done to detect NFAT1, activation of ERK1/2 and Akt in cell lysates. The results showed that blocking activation of ERK1/2 by U0126 promotes the expression of NFAT1. In contrast, blocking the activation of Akt by LY 294002 dramatically reduced the expression of NFAT1. One of 3 representative experiments is shown.