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. 2014 Jul 10;6(7):2024–2040. doi: 10.3390/toxins6072024

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effect of the heat treatment and proteinase K on the toxicity of protein extracted from PtC1015 clone. (A) The mortality rate was affected by the digestion of proteinase K. ●, Soluble fraction without proteinase K digestion; ■, Insoluble fraction without proteinase K digestion; ○, Soluble fraction with proteinase K digestion; □, Insoluble fraction with proteinase K digestion. (B) The mortality rate was affected by heat treatment. ●, Soluble fraction without heat treatment; ■, Insoluble fraction without heat treatment; ○, Soluble fraction with heat treatment; □, Insoluble fraction with heat treatment. All experiments were repeated at least three times and values were presented with standard deviation.