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. 2014 Jul 10;6(7):2024–2040. doi: 10.3390/toxins6072024

Table 1.

Specific primer sets for amplifying the various toxin genes components.

Names of the Target Genes Primers Primer Sequence (5'→3')
Partial tccB gene (0.46 kb) TccB1
TccB2
GTG ACT CAG CTT TCA ATC GCC AT
GAT AGA TCC GCC ACC ATA TCC AG
Partial tcc gene locus (0.84 kb) Tcc1
Tcc2
AAR YTG GCT GAA GAG CAY
GTG CAA TAC CCT TAC CTG TGC
Partial tcd gene locus (0.77 kb) Tcd1
Tcd2
AAG ACC GTT TTT CCC GTT ATG AAT A
ATC ACC GGA TTG CAC CAC ATG
Partial tca gene locus (1.6 kb) Tca1
Tca2
GGA ATT CAT GCT GAG CTA TTG GCA A
GGT CGA CGC GAA TGG TAT AGC GAA T
Partial tcdA gene locus (2.2 kb) TcdA1
TcdA2
ACC GAT GGA TTT CAG TGG CGC T
GCG TCG ACT TGG CGA ATG GTG TAG CGA A
Partial chitinase gene (1.6 kb) ChiA1
ChiA2
GCG AAT TCC ATA TGT TTA AAA CAA TTG TAT CG
CTG AAA GTA CAG GTT CTC ATT TAA TTT GCA CGC TTC ACA
Partial mcf gene locus (0.87 kb) Mcf1
Mcf2
CCT GAC GCC GTT GCC CGA TGA CAC
AAC AGG GCA CCG GAT TCA GAG ATG