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. 2014 Jul 14;6(7):2050–2063. doi: 10.3390/toxins6072050

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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B. thuringiensis 4A4 produces crystal proteins. (A) Light microscopy image of Coomassie-stained spore-crystal mixture of B. thuringiensis 4A4. Spores are unstained (white arrow), black structures are crystal proteins (black arrow). Scale bar is 20 μm; (B) Scanning electron microscopy image of spore-crystal mixture of B. thuringiensis 4A4. Bi-pyramidal crystals are shown (pointed with white arrow); (C) SDS-PAGE profile of B. thuringiensis 4A4 crystal proteins. Dominant protein of around 140 kDa is shown.