Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 22;6(7):2162–2176. doi: 10.3390/toxins6072162

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

pH-dependent efficacy of autoproteolytic cleavage of toxin A (TcdA) and TcdA fragments. (A) Inositol-hexakisphosphate (IP₆)-induced cleavage of TcdA (308 kDa) and TcdA^1–1065 (120 kDa) at pH 5.0 and pH 7.4. The TcdA-specific immunoblot illustrates unprocessed toxins and the cleaved glucosyltransferase domain (62 kDa); (B) Western blot (upper panel) and densitometric analyses (lower panel) reflecting the cleaved GT-domain of TcdA and TcdA^1–1065 in dependence of pH. Data are presented as means ± SD (n = 3).