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. 2014 Jul 22;6(7):2162–2176. doi: 10.3390/toxins6072162

Figure 2.

Figure 2

TcdA located combined repetitive oligopeptides (CROPs) facilitate the binding of TcdA1–1874 to HT29 cells. Flow cytometry analysis shows the binding of fluorescently (Atto488)-labeled TcdA1–1874 to HT29 cells (green curve) and to HT29 cells preloaded with TcdA CROPs (red curve). The blue peak with the black line represents the autofluorescence of HT29 cells in the absence of labeled TcdA1–1874. The excitation and emission wavelengths of the fluorophore are 501 nm and 523 nm, respectively, setting the band-pass width to 10 nm.