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TcdB CROPs do not facilitate the uptake of TcdB1–1852. Western blots showing the level of non-glucosylated Rac1 (upper panel) as a marker for the intracellular action of TcdB in HT-29 cells in triplicate. β-Actin (lower panel) served as the loading control. Rac1 glucosylation by CROP-truncated TcdB1–1852 was not altered in dependence of the pre-incubation of cells with the CROPs of TcdA or TcdB, respectively.
