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. 2014 Jul 23;6(7):2194–2209. doi: 10.3390/toxins6072194

Figure 2.

Figure 2

Specific transcription of LM1212 cry gene promoter in non-sporulating cells of Bt strains. (A) The LM1212 cry gene promoter drives transcription specifically and strongly in strain LM1212 in a subpopulation of crystal-producers; (B) The LM1212 cry gene promoter in strain HD73 drives transcription specifically and weakly in a small non-sporulating subpopulation of cells. Left: GFP/FM®-4-64 overlay. Right: bright field image. Sporulating cells are indicated by arrows and GFP-expressing cells are indicated by arrow heads. Cells were grown in SSM medium until T20 with erythromycin. The cell walls were stained with FM®-4-64.