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. 2014 Jul 9;12(7):4126–4147. doi: 10.3390/md12074126

Figure 1.

Figure 1

MALDI-TOF mass spectra of E. coli lipid A. Purified lipid A was prepared by mixing with ATT matrix as described in Materials and Methods. Spectral data were collected by Shimadzu Axima Confidence MS with a power setting of 65 and pulsed extraction at 2000 Da as the average of 581 profiles in the linear negative mode (A) or 1143 profiles in the linear positive mode (B). Peak interpretation is shown on the spectra, and in Table 1 with comparison to predicted molecular weights. To aid structural interpretation, the inset to (A) includes a summary of critical nomenclature and that of (B) shows the positive ion carbohydrate fragmentation pattern typical of lipid A molecules.