Figure 5.

Ascophyllan induces IFN-γ-producing T cells in an IL-12-dependent manner. Anti-IL-12p35 or control IgG Ab were injected intraperitoneally (i.p.) to C57BL/6 mice that had received prior i.v. injection of ascophyllan. (A) Intracellular IFN-γ expressions in CD4⁺ or CD8⁺ T cells were analyzed by flow cytometry (upper panel) and mean percentage of IFN-γ-positive cells were shown (lower panel); (B) IFN-γ production levels in sera were measured by ELISA. All data are representative of six samples from three independent experiments.