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. 2014 Jul 15;12(7):4214–4230. doi: 10.3390/md12074214

Figure 3.

Figure 3

Effects of fucoxanthin treatment on Akt and its related protein. (A) Cells were incubated with 20 μM fucoxanthin for various amounts of time (0–12 h). Cell lysates were prepared and Western blotting was performed with anti-Akt and anti-phospho Akt antibodies. * indicates significantly different from control cells (p < 0.05); After treatment with LY294002, cell lysates were subjected to electrophoresis (B) with anti-Akt, anti-phospho Akt. * indicates significantly different from control cells (p < 0.05) and # significantly different from fucoxanthin-treated cells (p < 0.05); (C) with anti-Nrf2 and anti-phospho Nrf2. * and ** indicates significantly different from Nrf2 and phospho Nrf2 of control, respectively (p < 0.05), # and ## indicates significantly different from Nrf2 and phosphoNrf2 of fucoxanthin-treated cells, respectively (p < 0.05); (D) with anti-GCLC and anti-GSSantibodies. * and ** indicates significantly different from GCLC and GSS of control, respectively (p <0.05), # and ## indicates significantly different from GCLC and GSS of fucoxanthin-treated cells, respectively (p < 0.05).