Figure 3.

Conventional features of intestinal and adaptive immunity are intact in the absence of eosinophils. (A) Median number of Peyer’s patches with individual data shown. (B) Mean intestinal wash IgA with individual data shown. (C) i.g. glucose challenge and blood glucose over time. (D) Serum OVA-specific IgE at day 28 from mice i.g. fed OVA or PBS daily from day 0 to 5 before i.p. immunization on day 7 and 14 with OVA adsorbed to aluminum hydroxide. (E–G) WT or ΔdblGATA1 (GATA) mice were kept naive or administered PN+CT (E) s.c., (F) p.r., or (G) i.p. weekly for 4 wk. (E–G, left) Serum PN-specific (PN-) IgG1 and PN-IgE at week 5. (E–G, middle) Clinical anaphylaxis assessment of hypothermia and vascular leakage after i.p. challenge at week 5. (E–G, right) Th2 cytokine production from PN-stimulated splenocytes. Mean ± SEM, n = 3–12 from 2–3 experiments. All WT versus GATA comparisons in this figure were not statistically different (ns).