Figure 2.

Gene expression changes and alternative activation of Gata6^ΔMac macrophages. (A) Scatter plots depict mRNA transcripts significantly decreased (left, blue) or increased (right, red) in Gata6^flox/flox (Ctrl) versus Gata6^ΔMac mice. (B) Expression of macrophage polarization and activation markers and eosinophil counts in the peritoneum. (C) Peritoneal B1-a and T lymphocyte counts. (D) Oxygen consumption rates (OCR) of Gata6^flox/flox and Gata6^ΔMac mice. (E) Mass spectrometric analysis of metabolites. (F) As shown in Fig. 1 D (blue line in flow cytometry gate), Gata6^ΔMac peritoneal macrophages were sorted into two populations based on a retention or reduction of the originally high levels of F4/80, then stained for nuclear Gata6. Bar, 5 µm. Various markers on Gata6^flox/flox (light gray histograms) and Gata6^ΔMac macrophages with preserved expression of Gata6 (Gata6⁺, from F4/80^high gate; gray histograms) and Gata6^ΔMac with efficient deletion of Gata6 (Gata6⁻ from F4/80^reduced gate; black histograms) were compared. (G) Analysis of similar markers on spleen or lung macrophages. Data in the figure summarize results from three or more independent experiments with two to five replicates per experimental group. Metabolic analysis was performed using five separate pools of sorted macrophages for each genotype. *, P < 0.05; **, P < 0.001 relative to controls, assessed using two-tailed Student’s t tests.