Figure 3.

SBF-SEM shows example of MDM-initiating demyelination at EAE onset. (A) Representative MDMs encircles the axoglial unit. A myelin ovoid within an intracellular phagolysosome shows physical continuity with myelin remaining attached to an axoglial unit which is undergoing active demyelination. In serial images, disrupted myelin shows continuity from outside to inside the MDM. (B) Rotated view from B demonstrating MDM-extensive attachment to axoglial unit and MiDM nearby with limited attachment to axon. A, axon; m, myelin; c, cytosol; n, nucleus. Red, MDM cytosol; green, MiDM cytosol; yellow: nuclei; blue, myelin and myelin debris; gray, axoplasm; red line, MDM plasma membrane. We studied 14 lesions from 7 EAE onset mice from 3 EAE inductions as follows: 8–10 mice were immunized at each experiment and 2–3 EAE onset mice were selected from each induction. Bar, 2 µm.