Figure 4.

MDMs surrounded, apposed, and invaded nodes of Ranvier at EAE onset. (A) SBF-SEM images and 3D reconstruction of SBF-SEM images of MDMs with a node of Ranvier. White and black arrow: microvillus. (B) SBF-SEM images and 3D construction of intratubal MDMs with demyelinated axon and node of Ranvier. (C) SBF-SEM images and 3D reconstruction of an MDM with intracellular myelin debris apposed to a node of Ranvier. Red, MDM cytosol; yellow, nucleus; blue, myelin; gray, axoplasm. M, myelin; c, cytosol. red line, MDM plasma membrane. We studied 14 lesions from 7 EAE onset mice collected as follows: 8–10 mice were immunized at each induction and 2–3 EAE onset mice were selected from each immunization. Bar, 2 µm.