Table I. Reaction rates of NADH oxidation titrated with antimycin A.
The deviation of Vtitr from 1 reflects the degree of pool function of cytochrome c (14).
Buffera plus | Vtitr (CRD1) | Vtitr (crd1Δ) |
---|---|---|
25 mM KPi | 1.07 ± 0.08 | 2.8 ± 0.3 |
25 mM KPi/50 mM TCAb | 3.0 ± 0.2 | 3.1 ± 0.4 |
Buffer was 0.6 M sorbitol, 1 mM EDTA, 1 mM MgCl2, pH adjusted to 7.0.
pH adjusted to 7.0.