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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Immunity. 2014 May 29;40(6):880–895. doi: 10.1016/j.immuni.2014.04.018

Figure 3. IKKε interacts with the C-terminal SPRY domain of TRIM6.

Figure 3

(A) Whole cell extracts (WCE) of HEK-293T cells co-transfected with HA-TRIM6 and either GFP-IRF3, FLAG-IKKε or TBK-1, were subjected to immunoprecipitation (IP) with anti-HA beads. (B) WCE of hDCs treated with SeV, LPS or IFNβ were subjected to IP with anti-IKKε antibody. (C) Localization of HA-TRIM6 and FLAG-IKKε in HeLa cells determined by confocal microscopy. (D) TRIM6 deletion mutants used for CoIP. (E) GST-pulldown of HEK-293T cells transfected with GST-TRIM deletion mutants and FLAG-IKKε. See also Figure S3.