TABLE 3.
Summary of Experiments1
| Mouse phenotype | Hybridized first | Hybridized second | Method of visualization |
|---|---|---|---|
| GAD67–GFP | Pomc-DIG | n/a | IHC (dαck-649) + HNPP |
| vGAT-EGFP | Pomc-DIG + vGat-FITC | n/a | IHC (dαck-649) + TSA Biotin Plus kit (SA-488) + HNPP |
| vGAT-EGFP | Gad67-FITC | n/a | IHC (dαck-649) + TSA Biotin Plus kit (SA-488) |
| POMC–EGFP | vGlut2-FITC | Gad65-DIG | IHC (dαck-649) + TSA Biotin Plus kit (SA-488) + HNPP |
| POMC–EGFP | Gad67-FITC | n/a | IHC (dαck-649) + TSA Biotin Plus kit (SA-488) |
| Wild type | vGlut1-DIG | n/a | HNPP |
1
Probes labeled with either digoxigenin (DIG) or fluorescein (FITC) were hybridized individually, at the same time, or sequentially. The target mRNA is listed alongside the phenotype of the animals used for each experiment, as well as a brief summary of the immunological methods used to detect each marker (GFP, DIG, or FITC). IHC, immunohistochemistry.