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The Vγ1 subset produces high levels of CCR5 ligands. γδ T cell subsets were sorted from naïve SJL/J splenocytes, plated at 1×106 cell/ml and stimulated on plate bound 2C11 (1ug/ml) overnight. RNA was isolated from cells and subjected to real time PCR analysis (a). Supernatant was collected and protein was analyzed by Cytokine Bead Array (b).
