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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Biomaterials. 2014 Jun 10;35(26):7346–7354. doi: 10.1016/j.biomaterials.2014.05.014

Figure 3.

Figure 3

CM adhesion, viability, metabolic activity, and maturation. A) Phase contrast images of CMs after one day of culture indicating enhanced CMs retention on 1.5% CNT-PG compared to PG scaffold. Quantified results of B) cell retention (day 1), C) live/dead assay (day 1) and D) normalized metabolic activity, confirming significantly improved cell survival and proliferation as a function of CNT content (*: P< 0.05). E) Quantified average preferred nuclei alignment between 0–10 degree and F) nuclei alignment distribution on the developed scaffolds after day 7 of culture, indicated significantly enhancement in cellular alignment as a function of CNT content (*: P< 0.05) (Random 1.5% CNT-PG scaffold was considered as control). Representative images of CMs proteins expression stained for G) Sarcomeric α-actinin (green), Cx43 (red) and DAPI (blue), and H) Troponin I (red) and DAPI (blue) after 7 days of culture, revealing organized sarcomeres with higher Cx43 expression on 1.5% CNT-PG than PG scaffold. A high magnification image (inset in Fig. 3G) shows interconnected sarcomeric structures perpendicular to the direction of the nanofibers.