Figure 4. Hypoxia activated NF-κB and immune response in Drosophila is IKK (Ird5) dependent.

(A) Wild-type (w1118) and IKK-deficient adult animals (Idr5) were exposed to 24 h 5% O₂ prior to total RNA extraction. Levels of Dorsal and Relish mRNA were analysed by QPCR using actin as a normalizing gene. Graph depicts mean and S.E.M. of a minimum of three independent experiments. Student's t test was performed and significance determined as follows:*P≤0.05, **P≤0.01, ***P≤0.001. (B). Wild-type (w1118) and IKK-deficient adult animals (Idr5) were exposed to 24 h 5% O₂ prior to total RNA extraction. Levels of the indicated immune response genes were analysed as in (A). Graph depicts mean and S.E.M. of a minimum of three independent experiments. Student's t test was performed and significance determined as follows:*P≤0.05, **P≤0.01, ***P≤0.001.