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. 2014 Jul 21;13:176. doi: 10.1186/1476-4598-13-176

Figure 6.

Figure 6

The suppression of KIF5B-RET kinase activity reduces the proliferation of positive lung cancer cells. A. Specific effects of ZD6474 on KIF5B-RET positive cells. A549 carrying KIF5B-RET cells and negative (A549, BEAS-2B and NCI-H1299) lung cell lines were seeded in 96-well plates, and then treated at different doses as indicated for 48 hours, and analyzed the IC50 by MTT assay. B. The suppression of KIF5B-RET kinase activity by ZD6474. The transformed KIF5B-RET A549 cells were treated either with DMSO or ZD6474, as indicated doses for 48 hours. The level of phosphorylated Tyr905 RET was analyzed by western blot. C & D. Effect of ZD6474 on the positive cells proliferation in vitro. A549 cells carrying KIF5B-RET or empty vector were seeded in 96-well plates and cultured for 72 hours, and proliferation rate was analyzed by MTT assay at the indicated time, and cellular morphology was obtained by light microscope at 48 hours. E. Effect of ZD6474 on the colony-forming ability of positive cells in vitro. A549 carrying KIF5B-RET cells were diluted and seeded in 6-well plates, treated with DMSO, ZD6474 8 μM or 16 μM for 14 days. The total number of colonies, each containing more than 40 cells, was determined (**P < 0.01, ***P < 0.001, Student’s t test).