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. 2013 Sep 2;33(30):3927–3938. doi: 10.1038/onc.2013.361

Figure 5.

Figure 5

Functional restoration of nuclear FOXO1 expression in ES cells results in impaired proliferation and reduced soft agar colony formation capability. Monolayer growth of (a) A673sh, and (b) SK-N-MC cells that were transfected with AKT- and CDK2-resistant FOXO1, or the empty vector control or FOXO1wt. Experiments were performed in triplicates and one representative experiment of at least three is shown. Corresponding controls for FOXO1 protein expression are shown below the growth curves. (c, d) Clonogenicity of A673sh and SK-N-MC cells as studied by soft agar colony formation assays in vitro. The corresponding colony sizes were not found to be drastically different between controls (empty vector and FOXO1wt) and nuclear FOXO1 mutants, suggesting significantly reduced clonogenicity triggered by nuclear FOXO1. Colonies were counted 14 days after seeding. **P<0.01 and ***P<0.001.