Table 1.
Detection of the derivative chromosomes in single S- and G0/G1 cells of three cell lines
|
A | |||||||
|---|---|---|---|---|---|---|---|
| dup(7p) 25.16 Mb (40 probes) | dup(9p) 24 Mb (42 probes) | del(18p) 8 Mb (28 probes) | dup(18p) 9.3 Mb (30 probes) | del(20p) a 1.7 Mb (8 probes) | |||
|
S_1_1 |
25 |
S_2_1 |
26 |
3a |
S_3_1 |
7 |
7 |
|
S_1_2 |
11 |
S_2_2 |
21 |
13 |
S_3_2 |
14 |
7 |
|
S_1_3 |
13 |
S_2_3 |
19 |
21 |
S_3_3 |
4a |
6 |
|
S_1_4 |
18 |
S_2_4 |
29 |
20 |
S_3_4 |
16 |
7 |
|
S_1_5 |
8a |
S_2_5 |
22 |
25 |
S_3_5 |
11 |
6 |
|
S_1_6 |
9a |
S_2_6 |
25 |
18 |
S_3_6 |
13 |
8 |
|
S_1_7 |
18 |
S_2_7 |
28 |
21 |
|
|
|
|
S_1_8 |
13 |
S_2_8 |
27 |
22 |
|
|
|
|
S_1_9 |
10a |
S_2_9 |
18 |
24 |
|
|
|
|
S_1_10 |
27 |
S_2_10 |
31 |
22 |
|
|
|
|
G0/G1_1_1 |
23 |
G0/G1_2_1 |
16 |
13 |
G0/G1_3_1 |
2a |
7 |
|
G0/G1_1_2 |
20 |
G0/G1_2_2 |
19 |
17 |
G0/G1_3_2 |
17 |
6 |
|
G0/G1_1_3 |
18 |
G0/G1_2_3 |
25 |
19 |
G0/G1_3_3 |
15 |
7 |
|
G0/G1_1_4 |
28 |
G0/G1_2_4 |
36 |
23 |
G0/G1_3_4 |
19 |
5 |
|
G0/G1_1_5 |
24 |
G0/G1_2_5 |
31 |
17 |
G0/G1_3_5 |
17 |
6 |
|
G0/G1_1_6 |
20 |
G0/G1_2_6 |
23 |
20 |
G0/G1_3_6 |
17 |
7 |
|
G0/G1_1_7 |
25 |
G0/G1_2_7 |
22 |
20 |
G0/G1_3_7 |
23 |
4 |
| |
|
G0/G1_2_8 |
31 |
19 |
|
|
|
|
B |
|
|
|
|
|
|
|
| |
Underestimation
b
|
No call
a
|
Total number of false negative |
Total number of false positive |
Total number of cells |
||
|
S |
14 |
3 |
17 |
5c |
26 |
||
| G0/G1 | 3 | 1 | 4 | 0 | 22 | ||
(A) Number of probes indicating the presence of an imbalance in the regions of interest according to the BAC array log2 intensity ratios of single cells per cell phase and cell line. In brackets the number of probes in the regions of interest. The number of probes indicating the presence of the imbalance of interest was significantly higher in G0/G1- compared to S-phase cells (Mann Whitney p = 0.029). (B) Total number of miss-calls per cell cycle phase. The accurate imbalance detection rate is significantly higher in G0/G1- compared to S-phase cells (Chi-square test p = 0.001).
aThe imbalance was not automatically indicated by the software.
bThe proximal or distal end of the imbalance was very weakly detected or not detected at all.
cThe log2 intensity ratios for 1 cell were indicative of a deletion in the control sample, while there is a duplication in the same region in the cell line of interest.