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. Author manuscript; available in PMC: 2015 Jun 3.
Published in final edited form as: Curr Protoc Chem Biol. 2014 Jun 3;6(2):117–133. doi: 10.1002/9780470559277.ch130206
Lectin Final Lectin Concentration
L-PHA (μg/ml) 0 10 15 20 22.5 25 30 35 40 60 75 100
WGA (μg/ml) 0 1 2 3 4 5 7.5 10 15 20 25 40
Con A (μg/ml) 0 1 3 5 7.5 10 15 20 25 30 40 50
RIC (ng/ml) 0 0.2 0.4 0.6 0.8 1 1.25 1.5 2.5 5 7.5 10
LCA (μg/ml) 0 5 7.5 10 15 20 25 30 40 50 75 100
The bolded concentrations are those at which ~10% wild-type CHO cells survive. This is defined as the D10 value. Note that RIC is ~1000-fold more toxic than the other lectins.