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. Author manuscript; available in PMC: 2015 Jul 10.
Published in final edited form as: Cell Rep. 2014 Jul 4;8(1):217–228. doi: 10.1016/j.celrep.2014.06.005

Figure 1. MEF2 is S-Nitrosylated In Vitro and In Vivo during Neurodegeneration.

Figure 1

(A) Excitotoxic NMDA (50 µM) was administered to cortical neurons in the presence or absence of NOS inhibitor l-NAME (500 µM). After 30 min, endogenous SNO-MEF2C was detected by biotin switch. SNO proteins were precipitated from neuronal lysates, and SNO-MEF2C detected with anti-MEF2C-specific antibody. Quantitative densitometry shown above immunoblots. Values are mean + SEM (n = 3 independent experiments, *p < 0.0001 by ANOVA with post-hoc Scheffé's test).

(B) SNO-MEF2C in contralateral or ipsilateral cortex 1 h after MCA occlusion. Values are mean + SEM (n = 3 animals, *p = 0.001 by t test).

(C) SNO-MEF2C in brains from WT littermates and Tg2576 AD mice. Values are mean + SEM (n = 4 animals in each group, *p = 0.001 by t test).

(D) SNO-MEF2C in brain tissues from human control or AD patients by biotin switch.

See also Figure S1.