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. 2014 Jul 29;8:83. doi: 10.3389/fncir.2014.00083

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Copyright © 2014 Albrecht, Dondzillo, Mayer, Thompson and Klug.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Examples of immunohistochemical label against GAD67 in the VNTB of GlyT2-GFP mice. (A–C) show a representative anti-GAD67 labeling pattern in the VNTB of a p14 mouse, with (A) being the overlay of both channels, (B) the antibody labeling in red and (C) the GlyT2-GFP expression in green. Images shown in (D–F) show the same labeling in an adult animal aged p70 (color coding is the same as in (A–C)]. Note that at p14, many glycinergic neurons are co-labeled with GAD67, while almost none of the glycinergic neurons seen at the older age are co-labeled against GAD67. All images are maximum projections of confocal stacks. Scale bar: 20 μm.