Skip to main content
. 2014 Jul 29;8:83. doi: 10.3389/fncir.2014.00083

Figure 2.

Figure 2

Glycinergic auditory nuclei in a brainstem of a p14 GlyT2-GFP mouse. (A) Maximum projection of 10 tiled confocal stacks through a 200 μm brain section cleared prior to imaging using the ClearT2-protocol. Five major nuclei with glycine label can be seen: the medial nucleus of the trapezoid body (MNTB), the ventral and lateral nuclei of the trapezoid body (VNTB and LNTB, outlined with white and red dashed lines, respectively), the SPN, and the ventral, high-frequency region of the lateral superior olive (LSO). (B,C) Closeup of the VNTB/LNTB area, showing the anatomical features used to discriminate between the VNTB and the LNTB. The image in (B) represents a more posterior location than (A) and (C) is located even more posterior than the ones in (A,B). Note the increasing gap between the two nuclei void of glycinergic cells. Scale bars: 100 μm.